In a collaborative context, some high purity recombinant proteins and polypeptides are available for research applications and for the development of enzyme immunoassays.

 

Bovine leukemia virus

Capsid protein p24

P24-tag polypeptide

 

Equine infectious anemia virus

Capsid protein p26 (sequence from an Argentine isolate)

P26-linker-tag polypeptide

 

Small ruminant lentivirus (CAEV / Maedi Visna)

Capsid protein p25 (genotype A)

Truncated p25 capsid protein (genotype B)

Truncated p25 capsid protein (genotype E)

P25-linker-tag polypeptide (genotype A)

Polypeptides of the exodomain of the transmembrane protein:

Polypeptide TM_1 (genotype A)

PolypeptideTM_2 (genotype B)

PolypeptideTM_1_2 multistrain (sequence corresponding to genotype A linked through a linker to a sequence related to genotype B)

PolypeptideTM_1_2-tag-S-tag

 

West Nile fever virus

WNVDIII Polypeptide (Glycoprotein E Domain III)

WNVDIII-linker-tag

Rift Valley fever virus

Nucleocapsid protein (South African isolate)

 

Blue Tongue Virus

Polypeptide derived from the VP7 protein

 

Argasid tick Ornithodoros moubata

Lipocalin protein

(this protein could be useful in studies relating to African swine fever).

 

The developed proteins have been used in the literature for the development of indirect ELISA tests.

Note: the “linker” indicates a short flexible amino acid sequence that serves to unite while keeping two functional domains apart (e.g. an antigenic protein from another antigenic protein, etc.).

By “tag” we mean an amino acid sequence that specifically binds streptavidin (Streptavidin-Binding Peptide (SBP)-Tag). Therefore polypeptides with this sequence can be recognized by streptavidin-peroxidase. This sequence is not patented. https://en.wikipedia.org/wiki/SBP-tag

 

With “S-tag” we indicate a peptide that binds the S-protein; therefore the polypeptideTM_1_2-tag-S-tag is recognized by both streptavidin and S-protein.

The “tagged” peptides can be useful in the immunochemical field (ELISA etc.).

 

The following tests are available:

-indirect ELISA test for CAEV/Maedi visna which in a preliminary analysis showed a sensitivity of 95% and a specificity of 96%.

 

-indirect ELISA for EIAV for the determination of anti-P26 protein antibodies (not validated).